Information for
Healthcare Providers

Direct Biologics is committed to providing the latest information on exosome scientific and clinical research. ExoFlo is an exosome allograft product isolated from bone marrow-derived MSCs that provides multiple regulatory proteins.

What are ExoFlo™ Exosomes?

Involved in cell to cell communication

Natural signals with no preservatives

Approximately 30nm – 150nm in size

...

Exosomes contain

More than 1,000 regulatory proteins1

  • Anti-inflammatory cytokines
  • Angiogenic growth factors
  • Matrix regenerative signals
  • Tumor suppressor signals
  • Inhibitors of apoptosis

Messenger RNA (mRNA) – ON SWITCH

Translated by recipient cells into new proteins that can regulate inflammatory cells and new vessel formation

Micro RNA (miRNA) – OFF SWITCH

May inhibit mRNA of chronic inflammatory cytokines and matrix catabolic enzymes

Exosomes Are Not Small Cells

.

  • ~1/1,000 the size of a cell
  • Do not have a nucleus
  • Cannot replicate
  • Do not have metabolic activity

Why Bone Marrow MSCs?

  • Peer reviewed published studies clearly indicate that BM-MSCs have a much higher safety profile and yield higher quality exosomes that deliver a more favorable profile of signaling factors vs. perinatal derived MSCs
  • Bone marrow derived MSCs are a much more characterized cell source.
    Over 63,000 peer reviewed papers have been published on bone marrow derived MSCs vs. up to 1,200 on perinatal derived MSCs.
  • Given the reported differences in safety, quality, and efficacy of exosome products, it is important for physicians to thoroughly investigate the exosome product being considered.

References

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Exosome Origins: Why the Cell Source Matters
Stem Cells Regen Med. 2020; 4(1): 1-4.
https://www.researchgate.net/publication/339136421_Exosome_Origins_Why_the_Cell_Source_Matters

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Direct Biologics, LLC Announces the Launch of ExoFlo Exosomes
published: 08 July 2019
https://www.newswire.com/news/direct-biologics-llc-announces-the-launch-of-exoflo-exosomes-20942231

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Current knowledge on exosome biogenesis and release description
Cell. Mol. Life Sci. (2018) 75:193–208
https://doi.org/10.1007/s00018-017-2595-9

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Exosomes Derived from Mesenchymal Stem Cells
Int. J. Mol. Sci. 2014, 15, 4142-4157; doi:10.3390/ijms15034142

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Extracellular vesicles potential roles in regenerative medicine
published: 03 December 2014
doi: 10.3389/fimmu.2014.00608

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Investigation of Content Stoichiometry Neural stem cells
PLOS ONE | DOI:10.1371/journal.pone.0146353 January 11, 2016

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Wound healing effect of adipose-derived stem cells A critical role of secretory factors on human dermal fibroblasts
Journal of Dermatological Science (2007) 48, 15—24

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Concise Review: MSC-Derived Exosomes for Cell-Free Therapy
Stem Cells 2017;35:851–858
https://doi.org/10.1002/stem.2575

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Mesenchymal Stem Cell Derived Secretome and Extracellular Vesicles for Acute Lung Injury and
Other Inflammatory Lung Diseases

Expert Opin Biol Ther . 2016 July ; 16(7): 859–871.
https://doi.org/10.1517/14712598.2016.1170804

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Paracrine Effects and Heterogeneity of Marrow-Derived Stem/Progenitor Cells: Relevance for the
Treatment of Respiratory Diseases

Сells Tissues Organs 2013;197:445–473
https://doi.org/10.1159/000348831

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      References
      1. Anderson, J.D., Johansson, H.J., Graham, C.S., Vesterlund, M., Pham, M.T., Bramlett, C.S., Montgomery, E.N., Mellema, M.S., Bardini, R.L., Contreras, Z., Hoon, M., Bauer, G., Fink, K.D., Fury, B., Hendrix, K.J., Chedin, F., EL‐Andaloussi, S., Hwang, B., Mulligan, M.S., Lehtiö, J. and Nolta, J.A. (2016), Comprehensive Proteomic Analysis of Mesenchymal Stem Cell Exosomes Reveals Modulation of Angiogenesis via Nuclear Factor‐KappaB Signaling. Stem Cells, 34: 601-613. doi:10.1002/stem.2298